0704-883-0675     |      dataprojectng@gmail.com

THE EFFECT OF METHANOL RHIZOME EXTRACT OF Nymphaea lotus LINN. (NYMPHAEACEAE) IN ANIMAL MODELS OF DIARRHOEA

  • Project Research
  • 1-5 Chapters
  • Abstract : Available
  • Table of Content: Available
  • Reference Style: APA
  • Recommended for : Student Researchers
  • NGN 3000

Abstract

Nymphaea lotus is one of the foremost aquatic macrophytes that have been identified in Nigerian fresh water bodies. It finds applications in the management of circulatory system disorders, digestive system disorders, infectionsand inflammations. This study aims to evaluate the antidiarrhoeal activity of the methanol rhizome extract of N. lotus Linn plant in laboratory animals. Shade-dried rhizome of N. lotus was extracted with 80% methanol using Soxhlet apparatus. Preliminary phytochemical screening was carried out.This revealed the presence of alkaloids, anthraquinones, carbohydrates, cardiac glycosides, flavonoids, saponins, steroids, tannins and triterpenes. The oral median lethal dose (LD50) was determined andacute toxicity test of the extract gave LD50 value greater than 5,000mg/kg p.o. in mice. The antidiarrhoeal activityof the methanol extract was determined in mice. The extract was screened for activity against castor oil-induced diarrhoea and magnesium sulphate-induced diarrhoea as well as effect on gastric transit time in mice. For castor oil-induced diarrhoea, the extract at doses of 200, 400 and 800mg/kg produced statistical significant reduction in the frequency of diarrhoea (at p<0.001, p<0.001 and p<0.01 respectively). The extract at 800 mg/kg produced a significant delay in onset of diarrhoea (p<0.05) comparable to loperamide (3mg/kg). The frequency of magnesium sulphate-induced diarrhoeawas also significantly reduced in the groups treated with 200, 400 and 800mg/kg of the extract at p<0.001, p<0.001 and p<0.01 respectively. At doses of 200mg/kg (76.5%) and 400mg/kg (72.6%), the protection produced comparable to loperamide, 3mg/kg (70.6%).All treated groups produced statistically significant reduction in the transit of charcoal meal along the intestinal tract at p<0.001. The standard antidiarrhoeal drug, atropine (5mg/kg) produced greater antimotility effect (56.83%) vi compared to the extract. Effect of methanol rhizome extract of N. lotus on isolated rabbit jejunum and guinea pig ileum was determined. The methanol rhizome extract ofNymphaea lotus at low concentration (4×10-4 – 6.4×10-2mg/ml) had contractile effect on the tone of contraction of the rabbit jejunum while at higher concentrations (8×10-2 -512×10-2 mg/ml) produced significant reduction in the tone and rate of spontaneous contraction of rabbit jejunum. Interaction of the extract,at concentration that causes relaxation, with acetylcholine,attenuated the effect of the latter in a manner similar to atropine.The methanol rhizome extract of N. lotus at lower concentrations (4×10-4 to 2×10-2 mg/ml) has no effect on contraction of the guinea pig ileum while higher concentrations (4×10-2 - 512×10-2 mg/ml) produced significant relaxant activity on guinea pig ileum. Interaction of the extract with histamine attenuated the effect of the latter which may be as a result of physiologic antagonism.This study has shown that the methanol rhizome extract of N. lotus has antidiarrhoeal properties thus justifying its use by the local population for this purpose.




FIND OTHER RELATED TOPICS


Related Project Materials

ASSESSMENT OF IMPACT OF DISCHARGED INDUSTRIAL EFFLUENTS ON PHYSICOCHEMICAL AND TRACE METAL POLLUTANTS OF ABA RIVER, ABA, NIGERIA

Statement of the Problem

The industrialization act of 1976 forced the doors open for the multiplication and establishment of small and me...

Read more
THE PITFALLS OF POVERTY ALLEVIATION PROGRAMMES IN THE CURBING THE INCIDENCE OF POVERTY IN RURAL AREAS

ABSTRACT

Poverty is general phenomenon in less developed countries including Nigeria. It is a c...

Read more
THE IMPACT OF MECHANICAL FARMING IN ECONOMIC DEVELOPMENT

Abstract

The purpose of this study is to investigate the impact of mechanized farming in the economic development of Ore...

Read more
INNOVATIONS IN TEACHING METHODOLOGIES IN TECHNICAL EDUCATION

Abstract: This study explores innovations in teaching methodologies in technical education...

Read more
THE CONCEPT OF WAR AND PEACE IN ISLAMIC LAW (MAFHUUM AL-HARB WAS-SALAAM FISHSHARI’ATILISLAMIYYAH)

ABSTRACT

This research work significantly examines a very interesting area of Islamic law. It is the portion of the Islamic law which rel...

Read more
THE IMPACT OF MODERN COMMUNICATION FACILITIES ON THE SECRETARY

Abstract

The secretary plays a very important role in the running of an organization. No matter how skillful and compete...

Read more
ABORTION AS A CAUSATIVE FACTOR TO THE INCREASE IN MORTALITY RATE AMONG ADOLESCENT AT AGES 13 to 20

ABSTRACT

This study is on abortion as a causative factor to the increase in mortality rate among adolescent in Plateau State of Nigeria....

Read more
EXAMINING THE EFFECTIVENESS OF MOBILE LEARNING APPLICATIONS IN VOCATIONAL EDUCATION

ABSTRACT: Examining the effectiveness of mobile learning applications in vocational education explores how digital tools can enhance learning...

Read more
GENDER INFLUENCE ON THE ACADEMIC PERFORMANCE OF STUDENTS’ IN ACCOUNTING AT THE SENIOR SECONDARY SCHOOL LEVEL

INTRODUCTION

The world has become a vastly complicated place. Scientific discovery, business development, technological...

Read more
ROLE OF A SECRETARY IN OFFICE ADMINISTRATION AND MANAGEMENT (A Case Study Of Ministry Of Finance Enugu)

ABSTRACT

This topic is the role of and management with particular reference to the ministry of finance, Enugu.

To carry out thi...

Read more
Share this page with your friends




whatsapp