THE EFFECT OF METHANOL RHIZOME EXTRACT OF Nymphaea lotus LINN. (NYMPHAEACEAE) IN ANIMAL MODELS OF DIARRHOEA
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
Abstract
Nymphaea lotus is one of the foremost aquatic macrophytes that have been identified in Nigerian fresh water bodies. It finds applications in the management of circulatory system disorders, digestive system disorders, infectionsand inflammations. This study aims to evaluate the antidiarrhoeal activity of the methanol rhizome extract of N. lotus Linn plant in laboratory animals. Shade-dried rhizome of N. lotus was extracted with 80% methanol using Soxhlet apparatus. Preliminary phytochemical screening was carried out.This revealed the presence of alkaloids, anthraquinones, carbohydrates, cardiac glycosides, flavonoids, saponins, steroids, tannins and triterpenes. The oral median lethal dose (LD50) was determined andacute toxicity test of the extract gave LD50 value greater than 5,000mg/kg p.o. in mice. The antidiarrhoeal activityof the methanol extract was determined in mice. The extract was screened for activity against castor oil-induced diarrhoea and magnesium sulphate-induced diarrhoea as well as effect on gastric transit time in mice. For castor oil-induced diarrhoea, the extract at doses of 200, 400 and 800mg/kg produced statistical significant reduction in the frequency of diarrhoea (at p<0.001, p<0.001 and p<0.01 respectively). The extract at 800 mg/kg produced a significant delay in onset of diarrhoea (p<0.05) comparable to loperamide (3mg/kg). The frequency of magnesium sulphate-induced diarrhoeawas also significantly reduced in the groups treated with 200, 400 and 800mg/kg of the extract at p<0.001, p<0.001 and p<0.01 respectively. At doses of 200mg/kg (76.5%) and 400mg/kg (72.6%), the protection produced comparable to loperamide, 3mg/kg (70.6%).All treated groups produced statistically significant reduction in the transit of charcoal meal along the intestinal tract at p<0.001. The standard antidiarrhoeal drug, atropine (5mg/kg) produced greater antimotility effect (56.83%) vi compared to the extract. Effect of methanol rhizome extract of N. lotus on isolated rabbit jejunum and guinea pig ileum was determined. The methanol rhizome extract ofNymphaea lotus at low concentration (4×10-4 – 6.4×10-2mg/ml) had contractile effect on the tone of contraction of the rabbit jejunum while at higher concentrations (8×10-2 -512×10-2 mg/ml) produced significant reduction in the tone and rate of spontaneous contraction of rabbit jejunum. Interaction of the extract,at concentration that causes relaxation, with acetylcholine,attenuated the effect of the latter in a manner similar to atropine.The methanol rhizome extract of N. lotus at lower concentrations (4×10-4 to 2×10-2 mg/ml) has no effect on contraction of the guinea pig ileum while higher concentrations (4×10-2 - 512×10-2 mg/ml) produced significant relaxant activity on guinea pig ileum. Interaction of the extract with histamine attenuated the effect of the latter which may be as a result of physiologic antagonism.This study has shown that the methanol rhizome extract of N. lotus has antidiarrhoeal properties thus justifying its use by the local population for this purpose.
Related Project Materials
KNOWLEDGE AND ATTITUDE OF EARLY CHILDHOOD CARE EDUCATION STUDENTS TOWARDS THE STUDY OF EARLY CHILD EDUCATION AS A COURSE
ABSTRACT
This study examined child labour in Edo State with focus on Benin City. This was examined on their everyday act...
DESIGN AND INSTALLATION OF 200 WATT SOLAR POWER SYSTEM
ABSTRACT
The 200W solar system was determined by load assessment, solar panel number determination, battery requirement and then inverter...
ASSESSMENT OF THE ROLE PERFORMANCE OF PRINCIPALS IN MANAGEMENT OF SECONDARY SCHOOLS IN ZAMFARA STATE, NIGERIA
ABSTRACT
This study focused on the Assessment oftherole performance of Principals in the Management of Secondary Schools in Zamfara State...
SOLAR TECHNOLOGY: AN ALTERNATIVE SOURCE OF ENERGY FOR NATIONAL DEVELOPMENT
ABSTRACT
This research was carried out to understand solar technology: An alternative source of energy for national deve...
OIL-PALM PRODUCTION IN EJIGBO COMMUNITY IN OSUN STATE, 1960-2000
-
- OBJECTIVE OF THE STUDY.
Indigenous arts in Nigeria are as old as time imm...
THE IMPACT OF VOCATIONAL CENTRES IN THE ATTAINMENT OF SELF-RELIANCE FOR WOMEN IN EDO STATE
ABSTRACT
This study attempts to analyze the effectiveness of vocational training centres in the attainm...
EFFECT OF COVID-19 ON THE FINANCIAL PERFORMANCE OF SMEs IN NIGERIA
ABSTRACT
Small and Medium Scale Enterprises (SMEs) are undeniably playing a significant role in the eco...
THE IMPACT OF TEAMWORK AND COLLABORATION ON EMPLOYEE MOTIVATION
Abstract: THE IMPACT OF TEAMWORK AND COLLABORATION ON EMPLOYEE MOTIVATION
Objective:...
AN APPRAISAL OF THE ENFORCEMENT OF HUMAN RIGHTS UNDER THE FUNDAMENTAL RIGHTS (ENFORCEMENT PROCEDURE) RULES 2009 IN NIGERIA
ABSTRACT
The Constitution of the Federal Republic of Nigeria 1999 chose certain rights to protect under chapter IV and termed them as Fun...
EFFECT OF DIVIDEND PAYMENT ON CORPORATE PERFORMANCE NIGERIAN BANKS
ABSTRACT
In this research work titled “Relationship between dividend payment and corporate perfor...
